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Estimation of action potential changes from field potential recordings in multicellular mouse cardiac myocyte cultures.
Halbach M, Egert U, Hescheler J, Banach K.
Institut fur Neurophysiologie, Universitat zu Koln.
Extracellular recordings of electrical activity with
substrate-integrated microelectrode arrays (MEAs) enable non-invasive
long-term monitoring of contracting multicellular cardiac preparations.
However, to characterize not only the spread of excitation and the
conduction velocity from field potential (FP) recordings, a more
rigorous analysis of FPs is necessary. Therefore in this study we aim
to characterize intrinsic action potential (AP) parameters by
simultaneous recording of APs and FPs. METHODS: A MEA consisting of 60
substrate-integrated electrodes is used to record the FP-waveform from
multicellular preparations of isolated embryonic mouse cardiomyocytes.
Simultaneous current clamp recordings in the vicinity of individual
microelectrodes and pharmacological interventions allowed us to
correlate FP and AP components and their time course. RESULTS: The
experiments revealed a linear relationship between AP rise time and FP
rise time as well as a linear relationship between AP duration and FP
duration. Furthermore a direct contribution of the voltage dependent
Na(+)- and Ca(2+)-current to the FP could be identified. CONCLUSION:
The characterization of the FP allows us for the first time to estimate
AP changes and the contribution of individual current components to the
AP by the help of non-invasive recording within a multicellular cardiac
preparation during long-term culture. Copyright 2003 S. Karger AG, Basel
PMID: 14586171 [PubMed - indexed for MEDLINE]