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A novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays.
Egert U, Schlosshauer B, Fennrich S, Nisch W, Fejtl M, Knott T, Muller T, Hammerle H.
und Medizinisches Institut (NMI), an der Universitat Tubingen in
Reutlingen, Markwiesenstr. 55, D-72770 Reutlingen, Germany.
coordinated activity of neural networks is crucial for brain
functioning. To understand the basis of physiological information
processing and pathological states, simultaneous multisite long-term
recording is a prerequisite. In a multidisciplinary approach we
developed a novel system of organotypically cultured rat hippocampal
slices on a planar 60-microelectrode array (MEA). This biohybrid system
allowed cultivation for 4 weeks. Methods known from semiconductor
production were employed to fabricate and characterize the MEA.
Simultaneous extracellular recording of local field potentials (LFPs)
and spike activity at 60 sites under sterile conditions allowed the
analysis of network activity with high spatiotemporal resolution. To
our knowledge this is the first realization of hippocampus cultured
organotypically on multi-microelectrode arrays for simultaneous
recording and electrical stimulation. This biohybrid system promises to
become a powerful tool for drug discovery and for the analysis of
neural networks, of synaptic plasticity, and of pathophysiological
conditions such as ischemia and epilepsy. Copyright 1998 Elsevier
Science B.V. All rights reserved.
PMID: 9630647 [PubMed - indexed for MEDLINE]