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Neural dynamics in cortex-striatum co-cultures--I. anatomy and electrophysiology of neuronal cell types.
Plenz D, Aertsen A.
Max-Planck-Institut fur biologische Kybernetik, Tubingen, Germany.
in vitro system was established to analyse corticostriatal processing.
Cortical and striatal slices taken at postnatal days 0-2 were
co-cultured for three to six weeks. The anatomy of the organotypic
co-cultures was determined using immunohistochemistry. In the cortex
parvalbumin-positive and calbindin-positive cells, which resembled
those seen in vivo, had laminar distributions. In the striatum,
strongly stained parvalbumin-positive cells resembling striatal
GABAergic interneurons and cholinergic interneurons were scattered
throughout the tissue. The soma area of these interneuron classes was
larger than the average striatal soma area, thus enabling visual
selection of cells by class before recording. Cortical neurons with
projections to the striatum showed similar morphological features to
corticostriatal projection neurons in vivo. No projections from the
striatum to the cortex were found. Intracellular recordings were
obtained from 94 neurons. These were first classified on the basis of
electrophysiological characteristics and the morphologies of cells in
each class were reconstructed. Two types of striatal secondary neurons
with unique electrophysiological dynamics were identified: GABAergic
interneurons (n = 17) and large aspiny, probably cholinergic,
interneurons (n = 15). The electrophysiological and morphological
characteristics of cortical pyramidal cells (n = 27), cortical
interneurons (n = 1), as well as striatal principal neurons (n = 34),
were identical to those reported for similar ages in vivo. Organotypic
cortex-striatum co-cultures are therefore suitable as an in vitro
system in which to analyse corticostriatal processing. The network
dynamics, which developed spontaneously in that system, are examined in
the companion paper.
PMID: 8848172 [PubMed - indexed for MEDLINE]